After 24 h, the cells were transfected using Lipofectamine 2000 Invitrogen with 4 Ојg of the pGL3 WT CTSO or pGL V CTSO constructs and 2 Ојg pRL CMV encoding a CMV driven renilla luciferase vector Promega, together with the carrier DNA pGL3 basic where buy cheap cytotec for sale I hope yours is coming soon, too